Table 1.

Strains and plasmids used in this study.

Strains and Plasmids	Description	Source
Strains
Aeromonas hydrophila
ZYAH72	Wild type	Lab collection
AHFGDS	ZYAH72 derivative, ΔastΔaltΔaphΔhlyΔaerA	This work
Escherichia coli
χ7213	thr-1 leuB6 fhuA21 lacY1 glnV44 recA1 ΔasdA4 Δ(zhf-2::Tn10) thi-1	[22]
Plasmids
pRE112	Suicide vector, sacB, mob-(RP4)R6K ori, Cmr	[23]
pRE112-ast	pRE112 derivative, designed for knockout of ast, Cmr	This work
pRE112-alt	pRE112 derivative, designed for knockout of alt, Cmr	This work
pRE112-aph	pRE112 derivative, designed for knockout of aph, Cmr	This work
pRE112-hly	pRE112 derivative, designed for knockout of hly, Cmr	This work
pRE112-aerA	pRE112 derivative, designed for knockout of aerA, Cmr	This work