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. 2021 May 2;9(5):448. doi: 10.3390/vaccines9050448

Figure 6.

Figure 6

Serum anti-peptide antibody levels of vaccinated GIA mice. Anti-peptide serum antibodies of GIA mice from study 2 (n = 8 mice per group) were assayed at a dilution of 1:1000 using biotinylated peptide-coated Neutravidin plates by ELISA. The X axis lists the biotinylated peptides used to coat the Neutravidin-containing wells. Peptide-bound IgG antibodies were detected using anti-mouse IgG secondary antibody. Optical Density (OD) values at 450 nm are shown on the Y axis. OD450 readings of the OVA peptide (background control) were subtracted from each of the other peptide-specific readings. One-way ANOVA with Tukey’s multiple comparison test were used for statistical analysis (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).