Skip to main content
. 2021 May 2;9(5):448. doi: 10.3390/vaccines9050448

Figure 7.

Figure 7

Intracellular (IC) cytokine and FoxP3 expression of spleen cells from control and LEAPS vaccine treated GIA mice. Spleen cells collected from the GIA mice at the end of study 2 were analyzed by flow cytometry for IC expression of cytokines or the Treg marker FoxP3. Expression data were calculated by multiplying mean fluorescence intensity (MFI) by the percent (%) of cytokine- or FoxP3 positive CD4+ T cells. (A) Ratios of anti-inflammatory (IL-4 or IL-10) to pro-inflammatory (IL-17A or IFN-γ) cytokines detected inside the GIA CD4+ cells. (B) IC FoxP3 expression in CD4+ spleen cells of each group of mice (MFI × %). Statistical analysis of the IC staining data was performed using (A) 2-way ANOVA with Fisher’s LSD test or (B) Kruskal–Wallis test with Dunn’s multiple comparison (n = 5 mice per group, * p ≤ 0.05).