Figure 8.

Cytokine production by spleen cells from control and LEAPS vaccine treated GIA mice. Spleen cells obtained from control or vaccinated GIA mice (n = 8 per group) from study 2 were either non-restimulated or restimulated with rhG1 antigen in culture for 4 days and then media samples were assayed for released cytokines. Cytokine results were obtained using Millipore’s Luminex MagPix instrument and R&D Systems’ Mouse Magnetic Luminex Assay kits (Biotechne brand). Results are presented as ratios of key anti-inflammatory to pro-inflammatory cytokines (to be comparable with the intracellular cytokine ratios shown in Figure 7) secreted by (A) Non-restimulated cell cultures and (B) Antigen-restimulated cell cultures (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). Statistical analysis was performed using one-way ANOVA and Fischer’s LSD multiple comparison tests. The concentrations of these four and a few additional cytokines are shown in Supplemental Figure S2.