Figure 4.

UV1-specific proliferation by T cells at different time points post-vaccination. UV1 peptide-specific proliferation was measured (counts per minute, cpm) at several sampling time points and representative examples are shown for patient N02 (A), patient N07 (B), patient N09 (C) and patient N11 (D). Proliferation was measured in response to peptide-loaded PBMC by ³H-thymidine incorporation. A 3-fold increase in proliferation compared to non-peptide control (T+ APC) was considered as an immune response. Superantigen SEC-3 stimulation was included as a positive control. Dotted line indicates cut-off for positive response, measurements until last positive time point is shown.