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. 2021 Apr 8;41(6):1987–2005. doi: 10.1161/ATVBAHA.121.316153

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© 2021 The Authors.

Arteriosclerosis, Thrombosis, and Vascular Biology is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Figure 2. — The involvement of Grb2 (growth factor receptor-bound protein 2), PI3K (phosphoinositide 3-kinase), or SHP2 (SH2 containing protein tyrosine phosphatase-2) in Gab2 (Grb2-associated binder2)-mediated inflammatory signaling. A, Human umbilical vein endothelial cells (HUVECs) were transfected with 200 nmol/L scrambled, Grb2, SHP2, or Gab2 siRNA for 48 h. The gene silencing was confirmed by immunoblotting. B, The transfected cells were treated with TNFα (tumor necrosis factor alpha), IL (interleukin)-1β (10 ng/mL), or lipopolysaccharide (LPS; 500 ng/mL) for 6 h. VCAM1 (vascular cell adhesion molecule 1) expression was analyzed by immunoblot analysis. Band intensities were quantified by densitometry, and the quantified data are shown in the right. C–H, HUVECs were left untreated (Unt), infected with control (C RV) or Gab2 retrovirus (Gab2 RV) were treated with LY294002 (5 µmol/L, LY) or SHP099 (0.5 µmol/L, SHP) for 1 h. Then, the cells were treated with TNFα (C and D), IL-1β (E and F), or LPS (G and H) for 6 h. The total RNA was extracted from the cells and MCP1 (macrophage chemoattractant protein 1; C, E, and G), IL-8 (D, F, and H) mRNA expression levels were measured by qRT-PCR. Results were expressed as relative expression (RE) to the control. One-way ANOVA was used to compare the data of experimental groups; Tukey post hoc multiple comparison test was used to obtain statistical significance. ns indicates no statistically significant difference. ***P<0.001, **P<0.01, *P<0.05, ***P<0.001.