Figure 1. . Flow cytometry panel from human peripheral blood.

After obtaining the sample, the red cells underwent lysis. The 14-color panel was performed on whole blood cells. Subpopulations of peripheral immune mononuclear cells (T cells, CD4⁺ T cells, CD8⁺ T cells, CD4⁻CD8⁻ T cells, CD4⁺CD8⁺ T cells, Tregs, B cells, NK cells, ILCs, basophils, MyDCs, pDCs, monocytes, classical [CD14⁺CD16⁻], intermediate [CD14⁺CD16^low] and nonclassical [CD14⁺CD16^hi] monocytes) were identified. The cellular gating depicted is representative of one healthy individual sample.

DC: Dendritic cell; FSC-A: Forward scatter area; FSC-H: Forward scatter height; HLA-DR: Human leukocyte antigen DR; ILC: Innate lymphoid cell; MyDC: Myeloid dendritic cell; NK: Natural killer; pDC: Plasmacytoid dendritic cell; SSC: Side scatter; Treg: Regulatory T cell; WBC: White blood cell.