Table 1. . Optimized combination of 14 commercially available fluorochrome-conjugated antibodies to reliably quantify 18 subpopulations of immune cells.
| Conjugation | Antibody | Clone (company) | Titer |
|---|---|---|---|
| FITC | Anti-human CD45 antibody | HI30 (BioLegend) | 1:50 |
| Percp-cy5.5 | Anti-human CD14 antibody | M5E2 (BD) | 1:50 |
| BV510 | Anti-human CD3 antibody | UCHT1 (BD) | 1:50 |
| Qdot655 | Anti-human CD4 antibody | S3.5 (Invitrogen) | 1:200 |
| PE-CY7 | Anti-human CD8 antibody | RPA-T8 (BD) | 1:100 |
| APC | Anti-human CD25 antibody | BC96 (BioLegend) | 1:20 |
| BUV395 | Anti-human CD16 antibody | 3G8 (BD) | 1:100 |
| BV605 | Anti-human CD56 antibody | NCAM16.2 | 1:100 |
| APC-CY7 | Anti-human HLA-DR antibody | L243 (BioLegend) | 1:100 |
| BV421 | Anti-human CD19 antibody | HIB19 (BD) | 1:100 |
| PE | Anti-human CD127 antibody | A019D5 (BioLegend) | 1:25 |
| BV710 | Anti-human CD123 antibody | 9F5 (BD) | 1:50 |
| Alexa Fluor® 700 | Anti-human CD11c antibody | Bu15 (BioLegend) | 1:50 |
| DAPI | Live/dead | 1:50 |