Figure 4.

FACS analysis of CD63 and CD86 expression on LDMs and HDMs. Freshly purified lung macrophages from the two subpopulations were suspended in PBS at a concentration of 5 × 10⁶/mL. Fifty microliters of cell suspension were incubated (4 °C, 20 min) with saturating amounts of FITC-conjugated anti-CD63 (Panel (A), closed histogram) or PE-conjugated anti-CD86 (Panel (B), closed histogram) Abs. Background fluorescence (Panels (A) and (B), open histogram) was assessed using isotypic control Abs. Quenching of macrophage autofluorescence and flow cytometry analysis were performed as described in Materials and Methods. The figure shows the results of one representative experiment of the six performed.