Table 2.
List of the primers used in the present study. The table reports the sequences of the primers used to amplify and sequence the 3 ORFV genes analyzed. The forward PPP3 primer was coupled with the reverse PPP4 primer to perform internal nested PCRs and amplify a 235 bp-long fragment of the B2L gene.
| Gene | Primer | Strand | Sequence | Size (bp) | Ta (°C) | Reference |
|---|---|---|---|---|---|---|
| B2L | PPP1 | Forward | 5′-gtcgtccacgatgcagct-3′ | 570 | 55 | [23] |
| PPP4 | Reverse | 5′-tacgtgggaagcgcctcgct-3′ | ||||
| PPP3 | Forward | 5′-gcgagtccgagaagaatacg-3′ | 235 | |||
| O45 | 045 F | Forward | 5′-cctacttctcggagttcagc-3′ | 400 | 47 | [24] |
| 045R | Reverse | 5′-gcagcacttctcctcgtag-3′ | ||||
| VIR | VIR1 | Forward | 5′-acaatggcctgcgagtg-3′ | 617 | 55 | [21] |
| VIR2 | Reverse | 5′-ttagaactgatgccgcag-3′ | ||||
| VIR 3 | Forward | 5′-tgatcaagtcgcctgca-3′ | 817 | 56 | Present study | |
| VIR 4 | Reverse | 5′-acaaatctcttgagcagct-3′ |