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. 2021 May 4;10(5):1101. doi: 10.3390/cells10051101

Figure 2.

Figure 2

MEK1/2-ERK inhibition is involved in LT-induced cell cycle arrest in B16-BL6 and HT-29 cells. (A) B16-BL6 and HT-29 cells were seeded in 96-well plates for 18 h. Cells were then exposed to U0126 (12.5 µM) or SB203580 (12.5 µM) for 24 h, and cell survival was measured by the MTT assay. (B) Similarly, HT-29 (top panel) and B16-BL6 (bottom panel) cells were seeded in 96-well plates for 6 h. Cell were then treated with U0126 (12.5 µM) with or without PCI-34051 (PCI) for the time indicated. Cell proliferation was measured by the MTT assay. (C) Resistance HT-29 cells were selected after treating U0126 for 72 h. The surviving cells were replated and re-exposed to U0126 for the time indicated. Cell survival was measured by the MTT assay. (D) HT-29 cells were transfected with HDAC8 siRNA or random siRNA for 18 h and then treated with U0126 (12.5 µM) for the time indicated. Cell proliferation was measured by the MTT assay. (A–D) Data are expressed as means and ± SD (n = 3; N.S., not significant; *, p < 0.05 by the Student’s t-test).