Figure 2.

Cell viability and neuronal differentiation of rNSCs grown on laminin-coated AuNC-aECMs with/without laser irradiation. rNSCs were seeded onto laminin-coated AuNC-aECMs with varying coverage densities of AuNCs. Cells were analyzed with immunocytochemistry at 16 days post seeding (A). The laminin-coated AuNC-aECMs were treated by laser irradiation with varying parameters (0.5–5.0 W/cm² for 1–5 min), and the temperature at the surface in wells containing 1 mL cell culture medium was measured by a thermocouple (B). rNSCs were seeded onto laminin-coated AuNC-aECMs, and after 4 days, the cells were treated with indicated laser irradiation (0.5–5.0 W/cm² for 1–5 min). After 24 h, the cells were collected, and then, cell viability assay was performed using flow cytometry (C). Values represent the means ± SDs of three independent experiments. * p values < 0.05 vs. the control group (laminin-coated glass coverslip).