Figure 7.

Assessment of heat shock protein 27, 70, and 90α expression in rNSCs. rNSCs were seeded onto laminin-coated AuNC-aECMs and after 4 days, the cells growing on laminin-coated AuNC-aECMs were treated with laser irradiation (2.0 W/cm² for 1 min). The cells were collected at indicated time points and analyzed with flow cytometry to measure Hsp 27, 70, and 90α expression level (A–C). For bulk heating experiments, laminin-coated glass coverslips were placed into a 24-well plate, and rNSCs were seeded. After 4 days, plates were submerged in a circulating water bath at 43 °C for 30 min. The cells were collected with varying time points; then, the heat shock protein 27, 70, and 90α expression was measured by flow cytometry. Values represent the means ± SDs of three independent experiments. # p values < 0.05 vs. laminin-coated glass coverslip. * p < 0.05 vs. laminin-coated AuNC-aECM in the absence of laser irradiation group.