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. 2021 May 5;13(5):1554. doi: 10.3390/nu13051554

Figure 1.

Figure 1

Adapted from Elango et al. [4]. As protein intake increases, so does the limiting amino acid and the oxidation of the indicator amino acid. This represents an increase in the incorporation of the indicator amino acid into newly synthesized proteins. A plateau in the oxidation of the indicator amino acid represents a maximal rate of incorporation in proteins. The breakpoint represents the estimated average requirement (EAR) for 50% of the population. The recommended dietary allowance (RDA) can be calculated as either 2 SD above the mean or the upper 95% CI. Created with BioRender.com.