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Fabricating gels of varying stiffness by changing the concentration of gel precursor solutions and culturing primary hepatocytes on soft and stiff PDMS gels. Primary rat hepatocytes were seeded on collagen PDMS substrates with physiologically relevant stiffness (2 kPa per soft mimics healthy liver tissue and 55 kPa per stiff mimics diseased/injured liver tissue). After one day in culture, fibroblast is seeded to establish coculture.
