Figure 4.

Study of cell viability after NC-T21 treatment dependent on pH. A549 and LLC1 cells were incubated with increasing concentrations of encapsulated T21 (NC-T21) or empty nanocapsules (NC) at pH 6.8 (blue) or 7.8 (black and grey) for 24, 48, and 72 h. (A). Cell viability was measured by triplicated MTT assay obtaining cell viability curves where IC₅₀ value was extrapolated. (B) Comparison of the IC₅₀ values. * p < 0.05. (C) IC₅₀ values (µg/mL ± SD), obtained from dose-response curves.