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Nanocapsules uptake by flow cytometry. A549 cells were incubated with DiO loaded amphoteric (NC-DiO) or anionic (NC-DiO-AN) NCs at pH 6.8 or pH 7.8 for 1, 15, 24, 48, and 72 h. Fluorescence intensity was measured by flow cytometry. (A) Mean intensity fluorescence is represented ± SD of three independent experiments. (B) Mean intensity fluorescence ratio at pH 6.8 respect to pH 7.8 is represented. **** p > 0.0001; ** p > 0.01; * p > 0.05 ns: not significant.
