Figure 4.

Phylogenetic relationships among Blastocystis partial SSU rRNA gene sequences of the barcoding region generated in the present study (represented with a black filled circle) and representative reference sequences of the accepted subtypes (Table 2). Proteromonas lacertae was used as outgroup taxon to root the tree. Analysis was conducted by a maximum likelihood method. Genetic distances were calculated using the Kimura two-parameter model. This analysis involved 64 nucleotide sequences, and there were a total of 587 positions in the final dataset. Bootstrap values lower than 75% are not displayed.