Figure 3.

Vaccination barely alters the immune response at transcriptional level in brain. Expression of immune-related genes (A) rtp3, (B) herc4, (C) mx, (D) mhcii and (E) tcrb in the brain of Senegalese sole specimens 7-, 15- and 30-days after intraperitoneal vaccination with low and high dosages (10⁵ and10⁷ TCID₅₀/mL, iSs160L and iSs160H, respectively) of BEI-iSs160 vaccine (inactivated with BEI) and form-iSs160 (formalin inactivated) or PBS (Control). Data represent the mean ± standard error of the mean (SEM; n = 6 fish/group and time). Lower letters denote statistical differences between groups at a same time-point (p < 0.05), whilst asterisks indicate those between time-points in the same group (* p < 0.05; ** p < 0.01).