Figure 4.

All vaccines induce partial protection and BEI-iSs160 decreases the viral load after challenge. (A) viral load (n = 3 fish/group and time) in brain and (B) Percent of survival during 45 days of in vivo infection in intraperitoneally vaccinated Senegalese sole specimens vaccinated with low and high dosages (10⁵ and 10⁷ TCID₅₀/mL, iSs160L and iSs160H, respectively) of BEI-iSs160 vaccine (inactivated with BEI) and form-iSs160 (formalin inactivated) or PBS (Control). Infection was performed by 3 h of immersion with 10⁷ TCID₅₀/mL. Survival rates were compared between groups using Kaplan Meier test. Lower letters denote statistical differences between groups of the same time point according to the two-way ANOVA test (p < 0.05).