Figure 6.
Vaccination mainly decreases the immune response upon NNV infection at a transcriptional level in head-kidney but not in brain. Expression of immune-related genes in (A) head-kidney and (B) brain of Senegalese sole specimens 45 days after 3 h of immersion with 105 TCID50/mL of Ss160 in fish previously vaccinated with PBS (Control) or with low and high dosages (105 and 107 TCID50/mL, iSs160L and iSs160H, respectively) of BEI-iSs160 vaccine (inactivated with BEI) and form-iSs160 (formalin inactivated) or PBS (Control). Data represent the mean ± standard error of the mean (SEM; n = 6 fish/group and time). Lower letters denote statistical differences between groups according to the two-way ANOVA test (p < 0.05).