Table 1.
Primer sequences used for gene expression analysis.
| Protein Name | Gene Name | Accession Number, UniGen Name or Reference | Sequence (5′–3′) | ||
|---|---|---|---|---|---|
| Nodavirus | RNA-dependent RNA polymerase | RNA1 | FJ803911 | F | TCCAAAAGAAAGAAGCATAC |
| R | TGGCATGTACCACGGAAC | ||||
| Senegalese sole | Receptor transporter protein | rtp3 | [20] | F | GACGCCCCAATGGTGGAT |
| R | CCAGATTCTTCATGAGGATGGTGAT | ||||
| E3 ubiquitin-protein ligase | herc4 | [20] | F | GCCAAAACACTGGCATGGTT | |
| R | AACGCCAAACAGGAAGTACCT | ||||
| Interferon-induced GTP-binding protein Mx | mx | [20] | F | CCTCTCTCCTTCAGGATCCTCCTCCTGTGC | |
| R | CAAAACAAGAAACTATCTGCCTGGTGGTTC | ||||
| Major histocompatibility complex II | mhcii | [20] | F | CGCTGATGAAAATGATCCACCTTCT | |
| R | ACCAGTCACATGACAGATCAGAGT | ||||
| T-cell receptor beta chain | tcrb | solea_v4.1_unigene681812 * | F | CAGGAGGCACAGCTATGAAA | |
| R | TCTCCACCCAAATCTCCAAA | ||||
| Beta actin | actb | DQ485686 | F | GACGACATGGAGAAGATC | |
| R | GGTGTTGAAGGTCTCAAA | ||||
* NOTE: UniGen transcriptomic database in http://www.scbi.uma.es/soleadb (accessed on 21 February 2021).