Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 May 20;153(6):e202012742. doi: 10.1085/jgp.202012742

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Szanto et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 3. — Effect of extracellular D₂O substitution on the activation kinetics of Shaker-IR. (A) The tsA201 cell expressing T449A Shaker-IR channels was repeatedly depolarized to +50 mV from a holding potential of −120 mV for 15 ms in outside-out configuration in the absence (black) or the presence of extracellular D₂O (red). The outward K⁺ current traces were fitted with a single-exponential function rising to a maximum according to the Hodgkin–Huxley n⁴ model (see Materials and methods). The activation time constant (τ_act) for a particular cell was defined as the average of time constants obtained for at least three depolarizing pulses repeated every 15 s in a sequence. (B) Bars and error bars indicate the mean ± SEM (n ≥ 4) of τ_act for the indicated clones. Symbols indicate individual data points (circles, T449A; down triangles, T449A/I470A; up triangles, T449K/I470C) obtained in H₂O//H₂O (filled symbols) and H₂O//D₂O (empty symbols). Asterisks indicate significant differences (*, P < 0.05; **, P < 0.01).