Skip to main content
. 2020 Dec 19;11(5):1227–1245. doi: 10.1016/j.apsb.2020.12.013

Figure 3.

Figure 3

SF3B3 silencing induces autophagy in vitro. (A)–(D) The MCF-7 cells transfected with si-NC, si-SF3B3 or long-term knockout SF3B3 and the expression of ATG5, ATG16L, SQSTM1/P62 and LC3 under normal or starvation conditions quantified by Western blot. ns, no significance; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. (E) Differential expression of LC3 in sh-NC/sh-SF3B3 and Baf A1 treated MCF-7 cells. Cell nucleus were stained with DAPI. Scale bar = 20 μm. ∗∗P < 0.01, sh-NC + Baf A1 vs. sh-NC; #P < 0.01, sh-SF3B3 + Baf A1 vs. sh-SF3B3; &&&&P < 0.0001, sh-SF3B3 vs. sh-NC.