Figure 2.

Celastrol protects mice from HFD-induced insulin resistance. (A)‒(D) Glucose tolerance test and insulin tolerance test were performed on HFD-fed mice treated with celastrol or vehicle. (E) Western blot analysis of insulin signaling in mice (Normal, HFD, HFD+Cel 3 mg/kg) liver. (F) Plasma glucose was determined in HFD-fed mice and celastrol treated mice. Data represent as mean ± SEM in (A) and (B) (n = 7), and (C)–(F) (n = 4). P values are determined by two-tailed Student's t test. ∗P < 0.05, ∗∗P < 0.01. GTT, glucose tolerance; ITT, insulin tolerance; AUC, calculated area under curve.