Figure 1.

SFN inhibited proliferation of ESCC cells. (A) After ECa109 and EC9706 cells were incubated with various concentrations (0, 10, 20, 40, 60 and 80 μmol/L) of SFN for 24, 48 and 72 h, viability of cells was determined by CCK-8 assay (n = 3). (B) Effects of SFN on proliferation of normal esophageal Het-1A cells (n = 3). (C) Clonogenic assay of cells treated with SFN (0, 5, and 10 μmol/L) for 7 days and the number of colonies in three independent experiments were quantified and statistically analyzed (n = 3). ∗∗∗P < 0.001 compared to control group.