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. 2021 May 6;11(5):1325. doi: 10.3390/ani11051325

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative mRNA expression levels of genes related to: (A) growth and development, (B) embryo-maternal communication, (C) methylation, and (D) apoptosis in fresh control (E7F and E8F) and stored embryos (E5C and E20C). E5C and E20C either did not express ESR1 or did not have enough samples; therefore, values were not considered for statistical comparison. Each dot represents a single embryo. The plot represents the descriptive variation (mean ± S.E.M., n = 6) within the group. Fold-changes were calculated with the comparative 2^−ΔΔCT method (* p < 0.05).