Table 1.

Primer for quantitative PCR.

Gene Symbol	Gene Name	NCBI/Ensembl Accession Number	Oligo Sequence (5′–3′)	Amplicon Length (bp)	PCR Efficiency (%)	R2 Value	Reference
ATP1A1	Equus caballus ATPase Na+/K+ transporting subunit alpha 1	NM_001114532.2, XM_023640223.1, XM_023640224.1	F: CTTGATGAACTTCAGCGCAAATA	104	94	0.990	This study
			R: GGTGTAAGGGCATTGGGA
			P: TGAGCCGAGGCTTAACAACTGCTC
BAX	Equus caballus BCL2-associated X protein	XM_014729721.1	F: AGGATGCGTCCACCAAGAAG	80	93.2	0.994	[37]
		XM_014729717.1	R: CCTCTGCAGCTCCATGTTACTG
			P: CTCAAGCGCATCGGAGATGAGCTG
BCL2	Equus caballus B-cell lymphoma 2	XM_001490436.2	F: TTGGAAAGCCTACCACTAATTGC	74	92.6	0.998	[37]
			R: CCGTGTTTATAGGCACAGGAGAT
			P: CCCACCTGAGCGGCTCCACC
CYP19A1	Equus caballus cytochrome P450 family 19 subfamily A member 1	NM_001081805.2	F: GGAGAGGAAACGCTCGTTATTA	107	99.2	0.999	This study
		XM_005602588.2	R: CCCATATACTGCAACCCAAATG
		XM_005602587.2	P: ATCACTACTCCTCCCGATTTGGCA
DNMT1	Equus caballus DNA methyltransferase 1	XM_014741825.1	F: GACCACCATCACGTCTCATTT	97	100.5	1	This study
			R: CTCCTCATCCACAGAATTGTCC
			P: AAACGGAAACCCGAGGAAGAGCTG
DNMT3A	Equus caballus DNA methyltransferase 3 α	XM_005600169.2	F: GATTATTGACGAACGCACAAGAG	112	100	0.998	This study
		XM_005600168.2
		XM_005600167.2
		XM_005600170.2	R: GTGTTCCAGGGTGACATTGA
		XM_005600171.2	P: TGCAAATGTCTTCGATGTTCCGGC
DNMT3B	Equus caballus DNA methyltransferase 3 β	XM_001916514.4	F: CGAGTCTTGTCCCTGTTTGAT	110	100.6	0.999	This study
			R: GCGATAGACTCTTCACACACTT
			P: CGCCACAGGGTACTTGGTTCTCAA
DNMT3L	Equus caballus DNA (cytosine-5-)-methyltransferase 3-like	XM_014736476.1	F: GCCCTCACTTGGTTGGTTT	98	100.5	0.999	This study
			R: CTTCCACACAGGCACAGTTT
			P: CAAAGTGCCCATCTGCTCTGGAGA
ESR1	Equus caballus estrogen receptor 1	NM_001081772.1	F: CACCCAGGAAAGCTCCTATTT	110	101.8	0.999	This study
			R: CGAGATGACGTAGCCAACAA
			P: TCCACCATGCCCTCTACACATTTCC
H19	Equus caballus H19, imprinted maternally expressed transcript	NR_027326.2	F: CCTCTAGCTCTGACTCAAGAATATG	103	94.3	0.992	This study
			R: CAGGTCCATCTGGTTCCTTTAG
			P: ACTCAGGAATCAGCTCTGGAAGGT
IGF1	Equus caballus insulin-like growth factor 1	NM_001082498.2	F: TGCTTCCGGAGCTGTGATCT	67	102	1	[34]
		XM_005606471.2
		XM_005606472.2
		XM_005606470.2	R: CCGACTTGGCAGGCTTGA
		XM_005606469.2	P: AGGAGGCTGGAGATGTACTGCGCACC
IGF2	Equus caballus insulin-like growth factor 2	NM_001114539.2	F: AAGTCCGAGAGGGACGTG	100	99.9	0.998	This study
			R: ATTGCTTCCAGGCGTTGT
			P: CCCGTGGTCAAGCTCTTCCAGT
NANOG	Equus caballus Nanog homeobox	XM_014740545.1	F: ACAGCCCCGATTCATCCA	72	102.3	0.999	[35]
			R: TCTTTGCCTCGCTCGTCTCT
		XM_001498808.1	P: CAGTCCCAGAGTAAAACCGCTGCCC
POU5F1	Equus caballus POU class 5 homeobox 1	XM_014734675.1	F: CGGGCACTGCAGGAACAT	73	100.8	0.999	[35]
			R: CCGAAAGAGAAAGCGAACTAGTATTG
		XM_001490108.5	P: TTCTCCAGGTTGCCTCTCACTCGGTTC
PSMB4	Equus caballus proteasome subunit beta type IV	XM_001492317.4	F: CTTGGTGTAGCCTATGAAGCCC	82	93.1	0.991	[38]
		XM_005610132.1
		XM_008515015.1
		XM_005613704.1	R: CCAGAATTTCTCGCAGCAGAG
PTGES2	Equus caballus prostaglandin-endoperoxide synthase 2	NM_001081775.2	F: GAGGTGTATCCGCCCACAGT	81	92.3	0.996	[36]
			R: AGCAAACCGCAGGTGCTC
			P: TCAGATGGAAATGATCTACCCGCCTCA
SNRPD3	Equus caballus small nuclear ribonucleoprotein D3 polypeptide.	XM_001489060.4	F: ACGCACCTATGTTAAAGAGCATG	120	99.4	0.996	[38]
		XM_008511652.1	R: CACGTCCCATTCCACGTC
SOX2	Equus caballus SRY box 2	XM_003363345.3	F: TGCGAGCGCTGCACAT	91	99.3	0.998	[35]
			R: AGCGTGTACTTATCCTTCTTCATGAG
			P: ATAAATACCGTCCTCGGCGGAAAACCAA

R²: correlation coefficient of standard curve; F and R: forward and reverse primer; P: hydrolysis probe.