Figure 2.

In vivo cytotoxicity induced by surv.VLP-SS-MUC1. (a) Timeline of cytotoxicity assay. On day 0, mice (n = 6 per treatment group) were vaccinated with VLPs delivering one or two antigens or empty VLP controls (VP60). 21 days later, mice received a boost with the same treatment and, on day 28, mice were injected with target cells, pulsed with either survivin or MUC1 peptide. Mice were culled 48 h after target cell injection, and splenocytes were analysed. (b) Specific lysis of target cells was calculated by comparing unpulsed to target cell ratio. Results show percent specific lysis of vaccinated treatment groups compared to the VP60 group ± SEM. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test, *** p < 0.001, ** p < 0.01, * p < 0.05.