Table 3.
List of helper T-cell epitopes with encountering MHC II alleles with their positional, prediction method, antigenicity, allergenicity, and toxicity information.
| Sl. No. | Peptide | MHC II Binding Allele | Start | End | Method | Percentile Rank | Vaxijen Score | Allergenicity | Toxicity |
|---|---|---|---|---|---|---|---|---|---|
| Wuhan Isolate | |||||||||
| 1 | MFVFLVLLPLVSSQC | HLA-DRB1*01:01 | 1 | 15 | Consensus | 0.24 | Antigen (0.5741) | Non-allergen | Non-toxic |
| 2 | MFVFLVLLPLVSSQC | HLA-DPA1*03:01/DPB1*04:02 | 1 | 15 | Consensus | 0.34 | Antigen (0.5741) | Non-allergen | Non-toxic |
| 3 | VLLPLVSSQCVNLTT | HLA-DRB4*01:01 | 6 | 20 | Consensus | 1.5 | Antigen (0.8957) | Non-allergen | Non-toxic |
| 4 | LHSTQDLFLPFFSNV | HLA-DPA1*01:03/DPB1*02:01 | 48 | 62 | Consensus | 1.4 | Antigen (0.2110) | Allergen | Non-toxic |
| 5 | LFLPFFSNVTWFHAI | HLA-DPA1*01:03/DPB1*04:01 | 54 | 68 | NetMHCIIpan | 0.81 | Antigen (0.2477) | Non-allergen | Non-toxic |
| 6 | KTQSLLIVNNATNVV | HLA-DRB3*02:02 | 113 | 127 | NetMHCIIpan | 0.17 | Antigen (0.6303) | Allergen | Non-toxic |
| 7 | SFVIRGDEVRQIAPG | HLA-DRB3*01:01 | 399 | 413 | Consensus | 0.51 | Antigen (0.5882) | Non-allergen | Non-toxic |
| 8 | GNYNYLYRLFRKSNL | HLA-DRB1*11:01 | 447 | 461 | Consensus | 0.22 | Non-antigen (0.1808) | Allergen | Non-toxic |
| 9 | PYRVVVLSFELLHAP | HLA-DPA1*03:01/DPB1*04:02 | 507 | 521 | Consensus | 0.25 | Antigen (0.8161) | Non-allergen | Non-toxic |
| 10 | FNFNGLTGTGVLTES | HLA-DRB1*09:01 | 541 | 555 | Consensus | 0.75 | Antigen (0.7797) | Non-allergen | Non-toxic |
| 11 | DIPIGAGICASYQTQ | HLA-DQA1*05:01/DQB1*03:01 | 633 | 677 | Consensus | 1.2 | Antigen (1.1088) | Non-allergen | Non-toxic |
| 12 | IAIPTNFTISVTTEI | HLA-DRB1*07:01 | 712 | 726 | Consensus | 0.47 | Antigen (0.7719) | Allergen | Non-toxic |
| 133 | CSNLLLQYGSFCTQL | HLA-DRB1*15:01 | 749 | 763 | Consensus | 0.58 | Antigen (0.6336) | Non-allergen | Non-toxic |
| 14 | WYIWLGFIAGLIAIV | HLA-DQA1*05:01/DQB1*03:01 | 1214 | 1228 | Consensus | 0.58 | Antigen (0.5770) | Non-allergen | Non-toxic |
| 15 | IWLGFIAGLIAIVMV | HLA-DQA1*05:01/DQB1*03:01 | 1216 | 1230 | Consensus | 0.51 | Antigen (0.6150) | Non-allergen | Non-toxic |
| England Variant | |||||||||
| 16 | FVFLVLLPLVSSQCV | HLA-DRB1*01:01 | 2 | 16 | Consensus | 0.24 | Antigen (0.7185) | Non-allergen | Non-toxic |
| 17 | KTQSLLIVNNATNVV | HLA-DRB1*13:02 | 113 | 127 | Consensus | 0.01 | Antigen (0.6303) | Allergen | Non-toxic |
| 18 | YRVVVLSFELLHAPA | HLA-DPA1*01:03/DPB1*04:01 | 508 | 522 | NetMHCIIpan | 0.95 | Antigen (0.7072) | Non-allergen | Non-toxic |
| 19 | VVLSFELLHAPATVC | HLA-DRB1*01:01 | 511 | 525 | Consensus | 0.03 | Antigen (0.8618) | Non-allergen | Non-toxic |
| 20 | DIPIGAGICASYQTQ | HLA-DQA1*05:01/DQB1*03:01 | 663 | 677 | Consensus | 1.2 | Antigen (1.1088) | Non-allergen | Non-toxic |
| 21 | PRRARSVASQSIIAY | HLA-DPA1*02:01/DPB1*14:01 | 681 | 695 | NetMHCIIpan | 1.2 | Non-antigen (0.2408) | Non-allergen | Non-toxic |
| 22 | YIWLGFIAGLIAIVM | HLA-DQA1*05:01/DQB1*03:01 | 1215 | 1229 | Consensus | 0.51 | Antigen (0.6090) | Non-allergen | Non-toxic |
| USA Variant | |||||||||
| 23 | SSGWTAGAAAYYVGY | HLA-DQA1*05:01/DQB1*03:01 | 255 | 269 | Consensus | 0.94 | Antigen (0.6604) | Non-allergen | Non-toxic |
| 24 | SGWTAGAAAYYVGYL | HLA-DQA1*05:01/DQB1*03:01 | 256 | 270 | Consensus | 1.2 | Antigen (0.6604) | Non-allergen | Non-toxic |
| 25 | VVVLSFELLHAPATV | HLA-DPA1*03:01/DPB1*04:02 | 510 | 524 | Consensus | 0.9 | Antigen (0.8083) | Non-allergen | Non-toxic |
| 26 | DIPIGAGICASYQTQ | HLA-DQA1*05:01/DQB1*03:01 | 663 | 677 | Consensus | 1.2 | Antigen (1.1088) | Non-allergen | Non-toxic |
| 27 | IAIPTNFTISVTTEI | HLA-DRB1*07:01 | 712 | 726 | Consensus | 0.47 | Antigen (0.7719) | Allergen | Non-toxic |
| 28 | RSFIEDLLFNKVTLA | HLA-DPA1*02:01/DPB1*05:01 | 815 | 829 | Consensus | 1.4 | Non-antigen (−0.0341) | Allergen | Non-toxic |
| 29 | GWTFGAGAALQIPFA | HLA-DRB1*09:01 | 885 | 899 | Consensus | 0.35 | Non-antigen (0.4665) | Non-allergen | Non-toxic |
| 30 | PREGVFVSNGTHWFV | HLA-DRB1*13:02 | 1090 | 1104 | Consensus | 1.2 | Antigen (1.0165) | Non-allergen | Non-toxic |
| 31 | REGVFVSNGTHWFVT | HLA-DRB3*02:02 | 1091 | 1105 | NetMHCIIpan | 0.2 | Antigen (1.0165) | Non-allergen | Non-toxic |
| 32 | SGNCDVVIGIVNNTV | HLA-DRB1*13:02 | 1123 | 1137 | Consensus | 1.3 | Antigen (0.5968) | Non-allergen | Non-toxic |
| 33 | CDVVIGIVNNTVYDP | HLA-DRB1*13:02 | 1126 | 1140 | Consensus | 0.7 | Antigen (0.7320) | Non-allergen | Non-toxic |
| 34 | WYIWLGFIAGLIAIV | HLA-DQA1*05:01/DQB1*03:01 | 1214 | 1228 | Consensus | 0.58 | Antigen (0.5770) | Non-allergen | Non-toxic |
| Indian Variant | |||||||||
| 35 | MFVFLVLLPLVSSQC |
HLA-DRB1*01:01 | 1 | 15 | Consensus | 0.24 | Antigen (0.5741) | Non-allergen | Non-toxic |
| 36 | DLFLPFFSNVTWFHA | HLA-DRB1*04:01 | 53 | 67 | Consensus | 1.1 | Non-antigen (0.2472) | Non-allergen | Non-toxic |
| 37 | KTQSLLIVNNATNVV | HLA-DRB1*13:02 | 113 | 127 | Consensus | 0.01 | Antigen (0.6303) | Allergen | Non-toxic |
| 38 | REFVFKNIDGYFKIY | HLA-DRB5*01:01 | 190 | 204 | Consensus | 0.17 | Non-antigen (−0.1712) | Allergen | Non-toxic |
| 39 | TRFASVYAWNRKRIS | HLA-DPA1*02:01/DPB1*14:01 | 232 | 246 | Consensus | 0.52 | Non-antigen (0.4963) | Allergen | Non-toxic |
| 40 | NYNYLYRLFRKSNLK | HLA-DRB1*11:01 | 448 | 462 | Consensus | 0.42 | Non-antigen (0.1089) | Allergen | Non-toxic |
| 41 | PYRVVVLSFELLHAP | HLA-DPA1*01:03/DPB1*02:01 | 507 | 521 | Consensus | 0.36 | Antigen (0.8161) | Non-allergen | Non-toxic |
| 42 | AIPINFTISVTTEIL | HLA-DRB1*07:01 | 713 | 727 | Consensus | 0.29 | Antigen (1.1305) | Non-allergen | Non-toxic |
| 43 | LQIPFAMQMAYRFNG | HLA-DRB4*01:01 | 894 | 908 | Consensus | 0.73 | Antigen (0.7205) | Non-allergen | Non-toxic |
| 44 | QQLIRAAEIRASANL | HLA-DPA1*02:01/DPB1*14:01 | 1010 | 1024 | NetMHCIIpan | 0.2 | Non-antigen (0.1269) | Allergen | Non-toxic |
| 45 | REGVFVSNGTHWFVT | HLA-DRB3*02:02 | 1091 | 1195 | NetMHCIIpan | 0.2 | Non-antigen (0.4461) | Allergen | Non-toxic |
| 46 | IWLGFIAGLIAIVMV | HLA-DQA1*05:01/DQB1*03:01 | 1216 | 1230 | Consensus | 0.51 | Antigen (0.6150) | Non-allergen | Non-toxic |
| South African Variant | |||||||||
| 47 | MFVFLVLLPLVSSQC | HLA-DRB1*01:01 | 1 | 15 | Consensus | 0.24 | Antigen (0.5741) | Non-allergen | Non-toxic |
| 48 | FVFLVLLPLVSSQCV | HLA-DRB1*01:01 | 2 | 16 | Consensus | 0.24 | Antigen (0.7185) | Non-allergen | Non-toxic |
| 49 | LHSTQDLFLPFFSNV | HLA-DPA1*01:03/DPB1*02:01 | 48 | 62 | Consensus | 1.4 | Non-antigen (0.2110) | Allergen | Non-toxic |
| 50 | KTQSLLIVNNATNVV | HLA-DRB1*13:02 | 113 | 127 | Consensus | 0.01 | Antigen (0.6303) | Allergen | Non-toxic |
| 51 | REFVFKNIDGYFKIY | HLA-DRB5*01:01 | 190 | 204 | Consensus | 0.17 | Non-antigen (−0.1712) | Allergen | Non-toxic |
| 52 | NITRFQTLLALHRSY | HLA-DRB5*01:01 | 234 | 248 | Consensus | 0.32 | Non-antigen (0.1775) | Non-allergen | Non-toxic |
| 53 | ATRFASVYAWNRKRI | HLA-DRB5*01:01 | 344 | 358 | Consensus | 0.49 | Non-antigen (0.3489) | Allergen | Non-toxic |
| 54 | NYNYLYRLFRKSNLK | HLA-DRB1*11:01 | 448 | 462 | Consensus | 0.42 | Non-antigen (0.1089) | Allergen | Non-toxic |
| 55 | PYRVVVLSFELLHAP | HLA-DPA1*02:01/DPB1*01:01 | 507 | 521 | Consensus | 0.3 | Antigen (0.8161) | Non-allergen | Non-toxic |
| 56 | IAIPTNFTISVTTEI | HLA-DRB1*07:01 | 712 | 726 | Consensus | 0.47 | Antigen (0.7719) | Non-allergen | Non-toxic |
| 57 | TSGWTFGAGAALQIP | HLA-DRB1*09:01 | 883 | 897 | Consensus | 0.34 | Non-antigen (−0.0178) | Non-allergen | Non-toxic |
| 58 | ALQIPFAMQMAYRFN |
HLA-DRB4*01:01 | 893 | 907 | Consensus | 0.81 | Antigen (1.0112) | Allergen | Non-toxic |
| 59 | QQLIRAAEIRASANL | HLA-DPA1*02:01/DPB1*14:01 | 1010 | 1024 | NetMHCIIpan | 0.2 | Non-antigen (0.1269) | Allergen | Non-toxic |
| 60 | REGVFVSNGTHWFVT | HLA-DRB3*02:02 | 1091 | 1105 | NetMHCIIpan | 0.2 | Non-antigen (0.4461) | Non-allergen | Non-toxic |
| 61 | CDVVIGIVNNTVYDP | HLA-DRB1*13:02 | 1126 | 1140 | Consensus | 0.7 | Antigen (0.7320) | Non-allergen | Non-toxic |
| 62 | YIWLGFIAGLIAIVM | HLA-DQA1*05:01/DQB1*03:01 | 1215 | 1229 | Consensus | 0.51 | Antigen (0.6090) | Non-allergen | Non-toxic |