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. 2021 May 6;9(5):464. doi: 10.3390/vaccines9050464

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Determination of the neutralising antibody response during the trial. The neutralising antibody titres after vaccination and challenge were evaluated by NPLA. Neg: The sample was negative for NPLA test; † symbol indicates that the animal was euthanized before this time point; asterisk symbol (*) indicates 21 dpv for groups A and B, and 5 dpv for groups C and D. Symbol (#) indicates animals that were euthanized at the day of challenge, according to the experimental design, see Materials and methods section.