Sequences of the Spanish 2020 WNV outbreak (pale blue); the closest relatives from previous outbreaks in Italy IT08, IT11, IT12-13 (dark blue); and the sequence JF719069 from a lethal equine case in Andalusia (Spain) in 2010. Other Spanish outbreaks were: JF707789, from a mosquito in Huelva, and FJ766331 and FJ766332, from a golden eagle in Toledo [14]. Other related outbreaks from the Mediterranean region (Cyprus MF797870) [13], or adjacent locations (United Arab Emirates KU588135 and Russia MN149538) are also included. Confidence intervals for the times at which the branching points occurred are marked with bars. Branching points are labeled for the discussion.