Figure 2.

HyPer7 is recycled by the thioredoxin system. (a,b) Strains HM123 (WT), AD88 (∆pgr1), SG154. (∆trx1), SB469 (∆grx1), SG152 (∆trr1), and MC184 (∆trx3), transformed with plasmid p728 expressing cytosolic HyPer7, were treated or not with H₂O₂ and oxidation of the reporter was estimated as described in Figure 1. (c) Scheme depicting the role of the thioredoxin system as the electron donor in HyPer7 recycling. (d) Strain EP160 (∆ctt1) transformed with plasmid p728 expressing cytosolic HyPer7 was treated or not with H₂O₂, and oxidation of the reporter was estimated as described in Figure 1. For each strain, average data from three biological replicates are shown, with error bars (S.D.) displayed in Figure S2.