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. 2021 May 11;12:671013. doi: 10.3389/fimmu.2021.671013

Figure 3.

Figure 3

Cytokine levels (pg/mL) including IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12(p70), IL-18, INF-γ, MCP-1, MIP-1α, MIP-1β, TNF-α, MIF and PAI-1 (A–N), analyzed in serum samples of the patient obtained on attendance to hospital date, on admission to Intensive Care Unit (ICU) date, on the day of the intravenous immunoglobulin (IVIG) plus 6-methylprednisolone (6MP) infusion, early (3 days) after the IVIG plus 6MP infusion and on mechanical ventilation removal. Cytokine measurements were retrospectively performed on -80 °C preserved samples, except for IL-6 (prospectively analyzed), using customized Milliplex® MAP kits (Panel A: IL-1α, IL-1β, IL-6, IL-8, IL-10, IL-12(p70), IL-18, INF-γ, MCP-1, MIP-1α, MIP-1β and TNF-α, ref. HCYTA-60K; Panel B: MIF and PAI-1, ref. HSP1MAG-63K; Merk-Millipore) according to manufacturer instructions on a Luminex platform with xPONENT vs. 4.2 as acquisition and analysis software.