Fig. 3. Dynamic diffusion of scaffold and client IDRs inside droplets. (a) FRAP recovery curves and images of three scaffold IDRs after 1 h of maturation. (b) Mobile fractions of scaffold and client IDRs inside the three IDR droplets (including DDX subcompartments) after 1 h of maturation. (c) FRAP recovery curves and images of client EGFP-DDX in IDR droplets including FUS droplets with EGFP-DDX subcompartments (Sub). Error bars: 1 s.d. (n = 15 from three independent experiments). Scale bars: 5 μm.