Fig. 5. Schematic illustration of the extraction of different species of RNA and evaluation of the purity of the isolated mRNA. (A) Schematic illustration of the extraction of different species of RNA. (B) Agarose gel electrophoresis-based purification. (C) Extracted-ion chromatograms of the standards of 4 modifications (m1G, m2G, t6A, and i6A). (D) Extracted-ion chromatograms of the 4 modifications detected in total RNA of HEK293T cells. (E) Extracted-ion chromatograms of the 4 modifications detected in mRNA of HEK293T cells. (F) Evaluation of the purity of the isolated mRNA by real-time quantitative PCR.
