Evaluation of the detection sensitivities of uridine, uridine modifications and guanosine upon CMCT labelling. Independent measurements of three samples were performed.
| Analytes | Without labelling (fmol, LODs) | With CMCT labelling (fmol, LODs) | Detection sensitivities improved fold |
|---|---|---|---|
| U | 160.0 ± 17.0 | 0.44 ± 0.04 | 364 |
| Ψ | 276.9 ± 22.1 | 2.20 ± 0.13 | 126 |
| m5U | 26.4 ± 3.2 | 0.29 ± 0.02 | 91 |
| m6U | 408.2 ± 28.1 | 0.29 ± 0.03 | 1408 |
| m1Ψ | 99.0 ± 10.3 | 0.31 ± 0.02 | 319 |
| hm5U | 145.8 ± 13.3 | 0.39 ± 0.03 | 374 |
| mo5U | 7.8 ± 0.8 | 1.28 ± 0.10 | 6 |
| mcm5U | 20.3 ± 4.2 | 0.54 ± 0.02 | 38 |
| G | 6.6 ± 0.6 | 0.90 ± 0.08 | 7 |