Figure 2.

Contents of (a) Progoitrin, (b) Glucobrassicin, (c) 4-Methoxyglucobrassicin, (d) Neoglucobrassicin, (e) total aGSLs and (f) total iGSLs in leaves of clubroot-resistant (mean of cultivars Aristoteles, Mendel, Creed) and -susceptible (mean of cultivars Bender, Ladoga, Visby) cultivars of Brassica napus 35 days post inoculation (dpi) after mock inoculation (control) or inoculation with either a less virulent isolate (P1, Hoisdorf) or a more virulent isolate (P1 (+), Grävenwiesbach) of Plasmodiophora brassicae. Contents represent the mean of six biological replicates, consisting of three plants each, from two independent experiments. Error bars represent the standard deviation. An analysis of variance (ANOVA) was performed to test the significance of main effects and interactions, and Tukey’s post hoc tests for the means of the factor variety were performed separately for inoculated and non-inoculated groups (for p-values see Supplementary Table S2). Different letters present significant differences between resistant and susceptible cultivars (a = p > 0.05; b = p < 0.05; c = p < 0.005). No significant differences were observed for other glucosinolates. DW, dry weight.