Table 1.

Overview of well-established protocols for generation PSC-KOs. The cell source, differentiation factors and culture methods are summarized and presented for each protocol.

Pluripotent Stem-Cell Source	Factors Used in Differentiation	Culture Method	Reference
Human embryonic stem-cells and human iPSCs	Y27632, CHIR99201, B27	Matrigel sandwich spheroids	Freedman et al. [42]
Human embryonic stem-cells and human iPSCs	CHIR99201, Noggin, Activin A, FGF9	96-well, low-attachment	Morizane et al. [43]
Mouse embryonic stem-cells and human iPSCs	Y27632, BMP4, FGF2, Activin A, CHIR99021, Retinoic Acid, FGF9	Transwell membrane	Taguchi et al. [44]
human iPSCs	CHIR99201, FGF9	Transwell membrane	Takasato et al. [45]