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The gene cluster related to acetoin biosynthesis in A. pasteurianus CICC 22518 (a), phylogenetic tree of AlsS1, AlsS2, and reported ALSs involved in acetoin biosynthesis (b), phylogenetic tree of AlsS2 and similar analogs in GenBank (c). The reconstruction was computed by the maximum likelihood method with bootstrapping test (1000 replicates) using MEGA X. GenBank accession numbers of the reference sequences were labeled in parentheses.
