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SDS-PAGE analysis of AlsS1 (a) and AlsS2 (b) expressed in E. coli. Lane M1, premixed protein marker (Broad) (TaKaRa, Dalian, China); lane 1, induced E. coli BL21-S1; lane 2, purified AlsS1; lane 3, purified AlsS2; lane M2, premixed protein marker (Low) (TaKaRa, Dalian, China); lane 4, induced E. coli BL21-S2.
