Table 1.

The bacterial strains and plasmids used in this study.

Strains and Plasmids	Genotype or Properties 1
Strains
Acetobacter pasteurianus CICC 22518	Wild strain, CmR
A. pasteurianus 22518-ΔalsS1	The alsS1-defective mutant of A. pasteurianus CICC 22518
A. pasteurianus 22518-ΔalsS2	The alsS2-defective mutant of A. pasteurianus CICC 22518
A. pasteurianus 22518-ΔalsR	The alsR-defective mutant of A. pasteurianus CICC 22518
Escherichia coli DH5α	F−, φ80d/lacZ ΔM15, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ−, thi-1, gyrA96, relA1
E. coli BL21(DE3)	F−, ompT, hsdSB (rB−mB−), gal (λ cl857, ind1, sam7, nin5, lacUV5-T7gene1), dcm (DE3)
E. coli HB101(pRK2013)	E. coli HB101 containing pRK2013 plasmid
E. coli BL21-S1	E. coli BL21(DE3) containing pET28S1 plasmid, KmR
E. coli BL21-S2	E. coli BL21(DE3) containing pET22S2 plasmid, AmpR
Plasmids
pET-22b(+)	Expression vector, AmpR
pET-28a(+)	Expression vector, KmR
pET28S1	Expression plasmid based on pET28a(+) for the alsS1 gene expression, KmR
pET22S2	Expression plasmid based on pET22b(+) for the alsS2 gene expression, AmpR
pKOS6b	Gene deletion vector, KmR, FCS
pKOS6b-ΔalsS1	Gene deletion plasmid based on pKOS6b for the alsS1 gene deletion, KmR, FCS
pKOS6b-ΔalsS2	Gene deletion plasmid based on pKOS6b for the alsS2 gene deletion, KmR, FCS
pKOS6b-ΔalsR	Gene deletion plasmid based on pKOS6b for the alsR gene deletion, KmR, FCS

¹ Cm^R: resistance to chloramphenicol; Km^R: resistance to kanamycin; Amp^R: resistance to ampicillin; FC^S: sensitivity to 5-fluorocytosine.