Figure 5.

Specific T-cell responses in splenocytes from mice immunized with chimeric VLPs measured by an ex vivo IFN-γ ELISPOT. Splenocytes were stimulated in vitro with parental RHDV VP60 (a), with FMDV O-UKG (b) or with a synthetic peptide encompassing the FMDV T-cell epitope (c). Each symbol represents the mean value of triplicates of splenocytes for an individual mouse. Horizontal lines represent the mean frequencies of IFN-γ-releasing cells for each animal group. Significant differences of each group as compared to the reference PBS group are shown as * p < 0.05, ** p < 0.01, *** p < 0.001.