Table 1.
Evidence for protection in animals immunized with the chimeric VLP L-B/N-T.
| Inoculum | Vaccine Dose (mg) |
Pig # | First Day with Vesicles 1 | Number of Vesicles 2 | Fever 3 | SN 4 Prime/Boost/Challenge |
Protection 5 |
|---|---|---|---|---|---|---|---|
| RHDV VLP L-B/N-T |
0.1 | #1 | 7 | 3 | 39.8 (4) | 1.9/2.4/3.4 | Partial protection |
| #2 | 7 | 1 | No fever | 1.5/2.2/3.6 | Full protection | ||
| #3 | 7 | 3 | 40.9 (4) | 2.0/2.3/3.6 | Partial protection | ||
| 1 | #4 | 4 | 4 | 40.1 (6) | 2.5/2.8/3.7 | Partial protection | |
| #5 | 7 | 3 | 39.9 (5) | 2.1/2.4/3.6 | Partial protection | ||
| #6 | 7 | 3 | No fever | 1.7/3.6/3.4 | Partial protection | ||
| Non-immunized | - | #7 | 4 | 4 | 40.7 (4) | 0/0/3.0 | Non-protected |
| #8 | 3 | 4 | 40.0 (4) | 0/0/2.9 | Non-protected |
1 First day post-challenge with detectable secondary lesions (vesicles); 2 total number of vesicles (vesicular lesions at inoculation site, feet, tongue, mouth and nose); 3 temperature (°C) and day post-challenge when maximum temperature was registered. No fever (temperature ≤39.5°); 4 serum neutralizing antibody responses reported as log10 TCID50 at days 19, 67 (48 days post-boost) and 77 (10 days post-challenge). Log10 SN values ≤0.6 are indicated as 0; 5 animals were considered protected when lesions were only observed at the infection site [38]; partially protected when animals showed mild and delayed signs of disease.