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. 2021 May 6;13(5):847. doi: 10.3390/v13050847

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Phylogenetic analysis of Hantaan virus (HTNV) whole-genome sequences by using amplicon next-generation sequencing (NGS) and MiSeq and MinION platforms. Phylogenetic trees were generated using maximum-likelihood method and bootstrap of 1000 iterations based on (A) HTNV L segment (1–6533 nt), (B) HTNV M segment (1–3616 nt), and (C) HTNV S segment (1–1696 nt). The numbers at each node are bootstrap probabilities, as determined for 1000 iterations. The newly obtained HTNV are shown in bold lettering and designated by color indicating the specific sites: blue, Jangam-ri of Pocheon; green, Dosin-ri of Yeoncheon; brown, Sang-ri of Yeoncheon; orange, Gwanu-ri of Cheorwon; red, Dochang-ri of Cheorwon; and violet, Pungsan-ri of Hwacheon. The newly acquired HTNV genomic sequences in this study are described in Table S1. The following other HTNV genome sequences were used in the analysis: HTNV Aa03-387 (L segment, KT934958; M segment, KT934992; S segment, KT935026), Aa05-331 (L segment, KT934962; M segment, KT934996; S segment, KT935030), Aa09-410 (L segment, KU207177; M segment, KU207185; S segment, KU207193), Aa09-948 (L segment, KT934966; M segment, KT935000; S segment, KT935034), Aa10-434 (L segment, KT934970; M segment, KT935004; S segment, KT935038), Aa10-518 (L segment, KT934971; M segment, KT935005; S segment, KT935039), Aa14-266 (L segment, KT934979; M segment, KT935013; S segment, KT935047), Aa14-272 (L segment, KT934980; M segment, KT935014; S segment, KT935048), Aa14-362 (L segment, KT934981; M segment, KT935015; S segment, KT935049), Aa14-368 (L segment, KT934982; M segment, KT935016; S segment, KT935050), Aa15-56 (L segment, KU207179; M segment, KU207187; S segment, KU207195), Aa15-58 (L segment, KU207180; M segment, KU207188; S segment, KU207196), Aa15-82 (L segment, MT012572; M segment, MT012560; S segment, MT012548), Aa15-84 (L segment, MT012573; M segment, MT012561; S segment, MT012549), Aa17-353 (L segment, MT012575; M segment, MT012563; S segment, MT012551), Aa17-367 (L segment, MT012576; M segment, MT012564; S segment, MT012552), Aa17-421 (L segment, MT012577; M segment, MT012565; S segment, MT012553), Aa17-422 (L segment, MT012578; M segment, MT012566; S segment, MT012554), Aa18-164 (L segment, MT012579; M segment, MT012567; S segment, MT012555), Aa18-179 (L segment, MT012580; M segment, MT012568; S segment, MT012556), HTNV 76-118 (L segment, NC_005222; M segment, M14627; S segment, M14626), HV004 (L segment, JQ083393; M segment, JQ083394; S segment, JQ093395).