. 2021 May 6;13(5):847. doi: 10.3390/v13050847

Table 3.

Results of MinION sequencing based on viral RNA copy number of Hantaan virus (HTNV) in the lung tissues of rodents collected from Gyeonggi and Gangwon Provinces, ROK, in 2019.

Viral RNA Copy Number (Copies/µL)	Sample	Site	Anti-HTNV IgG Titer	Nested RT-PCR	Ct Value	HTNV Genomes, % Coverage (Minimum Read Depth, >10×)
Viral RNA Copy Number (Copies/µL)	Sample	Site	Anti-HTNV IgG Titer	Nested RT-PCR	Ct Value	L Segment	M Segment	S Segment
10⁴ to 10⁵	Aa19-233	Cheorwon	1:64 ^a	Pos	20.5	96.8	98.5	100
10⁴ to 10⁵	Aa19-278	Cheorwon	1:32 ^a	Pos	22.2	92.9	98.4	100
10² to 10³	Aa19-236	Cheorwon	1:32 ^a	Pos	29.4	95.3	98.4	100
10² to 10³	Aa19-36	Cheorwon	1:512 ^b	Pos	29.7	91.7	98.4	100
10 to 10²	Aa19-89	Yeoncheon	1:64 ^a	Pos	31.8	95.9	98.4	100
	Aa19-57	Pocheon	1:8096 ^b	Pos	32.7	95.8	98.4	100
	Aa19-167	Yeoncheon	1:256 ^a	Pos	34.0	95.9	96.4	100
	Aa19-38	Cheorwon	1:16,384 ^b	Pos	34.4	92.3	98.4	100
	Aa19-153	Hwacheon	1:4096 ^b	Pos	34.5	92.7	98.4	100
1 to 10	Aa19-152	Hwacheon	1:512 ^a	Pos	36.4	82.6	97.0	92.1
1 to 10	Aa19-144	Hwacheon	1:256 ^b	Pos	37.3	88.8	96.4	95.1
0 to 1	Aa19-39	Cheorwon	1:512 ^b	Pos	40.0	65.4	90.6	92.2

HTNV: Hantaan virus; IgG, immunoglobulin G; nested RT-PCR, nested reverse transcription-polymerase chain reaction; Ct, cycle threshold; Aa, Apodemus agrarius; Pos, Positive; Neg, Negative; ^a, IFA test was performed from heart fluids; ^b, IFA test was performed from sera.