Table 4.

Sequencing error and consensus accuracy of amplicon-based MinION sequencing for Hantaan virus.

Viral RNA Copy Number (Copies/µL)	Sample	Site	Ct Value	L Segment				M Segment				S Segment
				Insertion	Deletion	Mismatch	Accuracy a	Insertion	Deletion	Mismatch	Accuracy	Insertion	Deletion	Mismatch	Accuracy
104 to 105	Aa19-233	Cheorwon	20.5	0	3	12	99.8	0	5	2	99.9	0	1	4	99.8
	Aa19-278	Cheorwon	22.2	0	4	9	99.9	0	6	2	99.9	0	1	0	100
102 to 103	Aa19-236	Cheorwon	29.4	0	5	9	99.9	0	6	3	99.9	0	1	1	99.9
	Aa19-36	Cheorwon	29.7	0	3	12	99.8	0	5	2	99.9	0	1	1	99.9
10 to 102	Aa19-89	Yeoncheon	31.8	0	3	6	99.9	0	5	6	99.8	0	1	1	99.9
	Aa19-57	Pocheon	32.7	0	4	9	99.9	0	5	4	99.9	0	1	2	99.9
	Aa19-167	Yeoncheon	34.0	0	4	8	99.9	0	4	4	99.9	0	1	0	100
	Aa19-38	Cheorwon	34.4	0	3	12	99.8	0	5	7	99.8	0	1	2	99.9
	Aa19-153	Hwacheon	34.5	0	5	14	99.8	0	4	3	99.9	0	1	3	99.8
1 to 10	Aa19-152	Hwacheon	36.4	0	5	18	99.7	0	2	11	99.7	0	1	7	99.6
	Aa19-144	Hwacheon	37.3	0	4	14	99.8	0	4	11	99.7	0	1	7	99.6
0 to 1	Aa19-39	Cheorwon	40.0	0	2	59	99.1	0	2	36	99.0	0	1	28	98.3
Total		Average (%)		0	3.8	15.2	99.8	0	4.4	7.6	99.8	0	1	4.7	99.7

Ct, cycle threshold; Aa, Apodemus agrarius; ^a, Percentage of nucleotide similarity between Oxford nanopore and Illumina MiSeq platforms.