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. Author manuscript; available in PMC: 2022 Mar 24.
Published in final edited form as: Chem Rev. 2021 Jan 28;121(6):3271–3296. doi: 10.1021/acs.chemrev.0c00967

Figure 15.

Figure 15.

RT interactions during the delayed chain termination (DCT) inhibition mechanism. Superposition of two RT structures with EFdA variants before and after translocation. Transparent light-brown sticks show the position of the DNA primer with ddG at the post-translocation P-site and EFdA-TP at the pretranslocation N-site (PDB 5J2M). After EFdA-MP is incorporated into the 3′-end of the primer and further elongated by a single nucleotide (RT/DNAEFdA-MPP• dTMPN), EFdA-MP is located at the P-site (PDB 5J2N). In this position, the 4′-ethynyl (4′-E) sterically clashes with the main chain carbonyl of Y183, and a large shift occurs only at the P-site of this primer to alleviate this negative interaction, as shown by the distance between the 4′ carbon atoms and the dihedral angles of the two structures. Based on ref 156.