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. 2021 May 25;10:e62503. doi: 10.7554/eLife.62503

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© 2021, Hertenstein et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 6. — (A–C`) Tret1-1 staining of the ventral nerve cord of starved and fed larvae expressing dominant-negative forms (InR^K1409A or InR^R418P) of the insulin receptor (InR) in the perineurial and subperineurial glial cells. (B, B`, C, C`) Tret1-1 levels in fed and starved animals expressing InR dominant negative are indistinguishable from wild type. (B`, C`) Tret1-1 upregulation upon starvation is seen in all cases. (D) Quantification of Tret1-1 upregulation in animals expressing InR^K1409A or InR^R418P. Shown is the ratio of relative Tret1-1 fluorescence intensity in the perineurial glial cells of starved versus fed animals. No significant differences between the genotypes are observed. N = 4, n = 12–16. (E–G`) Tret1-1 staining of the ventral nerve cord of starved and fed wild-type and Akh^−/− mutant animals (Akh^SAP or Akh^AP). Tret1-1 levels in fed and starved mutant animals are indistinguishable from wild type. (F`, G`) Tret1-1 upregulation upon starvation can be seen in all mutants. (H) Quantification of Tret1-1 intensities of Akh^SAP or Akh^AP. Shown is the ratio of relative Tret1-1 fluorescence intensity in the perineurial glial cells of starved versus fed animals. No significant differences are observed. N = 3, n = 5–8.

Figure 6—figure supplement 1. — (A–C`) Tret1-1 staining of the ventral nerve cord of starved and fed larvae expressing dominant-negative forms (InR^K1409A or InR^R418P) of the insulin receptor (InR) in the perineurial and subperineurial glial cells. (B, B`, C, C`) Tret1-1 levels in fed and starved animals expressing InR dominant negative are indistinguishable from wild type. (B`, C`) Tret1-1 upregulation upon starvation is seen in all cases. (D) Quantification of Tret1-1 upregulation in animals expressing InR^K1409A or InR^R418P. Shown is the ratio of relative Tret1-1 fluorescence intensity in the perineurial glial cells of starved versus fed animals. No significant differences between the genotypes are observed. N = 4, n = 12–16. (E–G`) Tret1-1 staining of the ventral nerve cord of starved and fed wild-type and Akh^−/− mutant animals (Akh^SAP or Akh^AP). Tret1-1 levels in fed and starved mutant animals are indistinguishable from wild type. (F`, G`) Tret1-1 upregulation upon starvation can be seen in all mutants. (H) Quantification of Tret1-1 intensities of Akh^SAP or Akh^AP. Shown is the ratio of relative Tret1-1 fluorescence intensity in the perineurial glial cells of starved versus fed animals. No significant differences are observed. N = 3, n = 5–8.