Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Drosophila melanogaster) | Tret1-1 | FBgn0050035 | ||
| Genetic reagent (D. melanogaster) | jebKK111857 | Vienna Drosophila Resource Center | v103047; FBgn0086677; FBst0474909 |
|
| Genetic reagent (D. melanogaster) | jebGD5472 | Vienna Drosophila Resource Center | v30800; FBgn0086677; FBst0458662 |
|
| Genetic reagent (D. melanogaster) | AlkGD42 | Vienna Drosophila Resource Center | v11446; FBgn0040505; FBst0450267 |
|
| Genetic reagent (D. melanogaster) | putKK102676 | Vienna Drosophila Resource Center | v107071; FBgn0003169; FBst0478894 |
|
| Genetic reagent (D. melanogaster) | putGD2545 | Vienna Drosophila Resource Center | v37279; FBgn0003169; FBst0461929 |
|
| Genetic reagent (D. melanogaster) | witKK100911 | Vienna Drosophila Resource Center | v103808; FBgn0024179; FBst0475666 |
|
| Genetic reagent (D. melanogaster) | saxGD50 | Vienna Drosophila Resource Center | v42457; FBgn0003317; FBst0464598 |
|
| Genetic reagent (D. melanogaster) | saxGD2546 | Vienna Drosophila Resource Center | FBgn0003317 | |
| Genetic reagent (D. melanogaster) | tkvKK102319 | Vienna Drosophila Resource Center | v105834; FBgn0003716; FBst0477660 |
|
| Genetic reagent (D. melanogaster) | Rab10GD13414 | Vienna Drosophila Resource Center | v28758; FBgn0015789; FBst0457628 |
|
| Genetic reagent (D. melanogaster) | Rab10GD16778 | Vienna Drosophila Resource Center | v46792; FBgn0015789; FBst0466897 |
|
| genetic reagent (D. melanogaster) | Rab10KK109210 | Vienna Drosophila Resource Center | v101454; FBgn0015789; FBst0473327 |
|
| Genetic reagent (D. melanogaster) | Tret1-1GD17787 | Vienna Drosophila Resource Center | v52360; FBgn0050035; FBst0469787 |
|
| Genetic reagent (D. melanogaster) | Rab7T22N | Bloomington Drosophila Stock Center | 9778; FBgn0015795 |
|
| Genetic reagent (D. melanogaster) | Rab10T23N | Bloomington Drosophila Stock Center | 9778; FBgn0015795; FBst0009778 |
|
| Genetic reagent (D. melanogaster) | Rab7EYFP | Bloomington Drosophila Stock Center | 62545; FBgn0015795; FBst0062545 |
|
| Genetic reagent (D. melanogaster) | Rab10EYFP | Bloomington Drosophila
Stock Center |
62548; FBgn0015789; FBst0062548 |
|
| Genetic reagent (D. melanogaster) | Rab19EYFP | Bloomington Drosophila Stock Center | 62552; FBgn0015793; FBst0062552 |
|
| Genetic reagent (D. melanogaster) | Rab23EYFP | Bloomington Drosophila Stock Center | 62554; FBgn0037364; FBst0062554 |
|
| Genetic reagent (D. melanogaster) | Rab7TRIP.JF02377 | Bloomington Drosophila Stock Center | 27051; FBgn0015795; FBst0027051 |
|
| Genetic reagent (D. melanogaster) | InRK1409A | Bloomington Drosophila Stock Center | FBgn0283499 | |
| Genetic reagent (D. melanogaster) | InRR418P | Bloomington Drosophila Stock Center | FBgn0283499 | |
| Genetic reagent (D. melanogaster) | UAS-dpp | Bloomington Drosophila Stock Center | 1486; FBgn0000490 |
|
| Genetic reagent (D. melanogaster) | CherrydsRNA | Bloomington Drosophila Stock Center | 35785; FBti0143385 |
|
| Genetic reagent (D. melanogaster) | UAS-CD8-GFP | Bloomington Drosophila Stock Center | 30002 or 30003 | |
| Genetic reagent (D. melanogaster) | AkhAP |
Gáliková et al., 2015
doi: 10.1534/genetics.115.178897 |
FBal0319564 | |
| Genetic reagent (D. melanogaster) | AkhSAP |
Gáliková et al., 2015
doi: 10.1534/genetics.115.178897 |
FBal0319565 | |
| Genetic reagent (D. melanogaster) | baboNIG8224R | Japanese National Institute of Genetics | FBal0275907 | |
| Genetic reagent (D. melanogaster) | gliotactin-Gal4 |
Sepp et al., 2001
Doi: 10.1006/dbio.2001.0411 |
– | |
| Genetic reagent (D. melanogaster) | repo-Gal4 |
Sepp et al., 2001
Doi: 10.1006/dbio.2001.0411 |
– | |
| Genetic reagent (D. melanogaster) | 46 F-Gal4 |
Xie and Auld, 2011
Doi: 10.1242/dev.064816 |
– | |
| Genetic reagent (D. melanogaster) | 9137-Gal4 |
DeSalvo et al., 2014
Doi: 10.3389/fnins.2014.00346 |
– | |
| Genetic reagent (D. melanogaster) | UAS-FLII12Pglu-700µδ6 |
Volkenhoff et al., 2018
Doi: 10.1016/j.jinsphys.2017.07.010 |
Maintained at S. Schirmeier lab | |
| Genetic reagent (D. melanogaster) | UAS-Gbb | P. Soba | – | |
| Genetic reagent (D. melanogaster) | UAS-RFP | S. Heuser | – | |
| Genetic reagent (D. melanogaster) | w1118 |
Lindsley and Zimm, 1992
ISBN 9780124509900 |
– | |
| Genetic reagent (D. melanogaster) | Tret1-1-stGFP | This paper | Maintained at S. Schirmeier | Tret1-1 promoter fusion to a nuclei-targeted GFP |
| Genetic reagent (D. melanogaster) | Tret1-1-Gal4 | This paper | Maintained at S. Schirmeier | Tret1-1 promoter induced Gal4 expression |
| Antibody | anti-Tret1-1 guinea pig polyclonal |
Volkenhoff et al., 2015 | Maintained at S. Schirmeier Lab | (1:50) |
| Antibody | anti-Laminin rabbit polyclonal | Abcam | ab11575 | (1:1000) |
| Antibody | anti-Repo mouse monoclonal | Developmental Studies Hybridoma Bank | 8D12 anti-Repo | (1:2) |
| Antibody | anti-GFP mouse monoclonal | Molecular Probes | A11120 | (1:1000) |
| Antibody | anti-GFP chicken polyclonal | Abcam | Ab92456 | (1:1000) |
| Antibody | anti-GFP JL-8 mouse monoclonal |
Clontech | Cat. 632381 | (1:10000) WB |
| Antibody | anti-Tubulin mouse monoclonal | Developmental Studies Hybridoma Bank | 12G10 anti-alpha-tubulin | (1:80) WB |
| Antibody | anti-Apontic rabbit polyclonal | Eulenberg and Schuh, 1997 | Gifted from Reinhard Schuh | (1:150) |
| Antibody | anti-Gbb mouse monoclonal | Developmental Studies Hybridoma Bank | GBB 3D6-24 | (1:20) |
| Recombinant DNA reagent | pBPGuw-stingerGFP (vector) | C. Klämbt | ||
| Recombinant DNA reagent | pBPGuwGal4 (vector) | Addgene | 17575 | |
| Recombinant DNA reagent | pGEM-He-Juel (vector) |
S. Bröer | ||
| Sequence-based reagent | Forward primer_Tret1-1prom | This paper | PCR primers | CACCGGTCTCAAGCTCTCTTTTTTGCCTTACATATTTT |
| Sequence-based reagent | Reverse primer_Tret1-1prom | This paper | PCR primers | TGGGTAAGTTGGAGAGAGAG |
| Sequence-based reagent | Forward primer Tret1-1 PA |
This paper | PCR primers | CGTCTAGAATGAGTGGACGCGAC |
| Peptide, recombinant protein | Reverse primer Tret1-1 PA | This paper | PCR primers | CGAAGCTTCTAGCTTACGTCACGT |
| Commercial assay or kit | pENTR/D- TOPO Cloning Kit | Thermo Fisher Scientific | K240020 | |
| Commercial assay or kit | mMESSAGE mMACHINE T7 Kit | Thermo Fisher Scientific | AM1344 | |
| Chemical compound, drug | 14C12-trehalose | Hartmann Analytic, Braunschweig | #1249 | |
| Chemical compound, drug | 14C6-glucose | Biotrend, Köln | #MC144-50 | |
| Chemical compound, drug | 14C6-fructose | Biotrend, Köln | #MC1459-50 | |
| Chemical compound, drug | Rotiszint eco plus scintillation cocktail | Carl Roth | Art. No. 0016.3 | |
| Software, algorithm | SigmaPlot | Jadel | SPSS Inc | |
| Software, algorithm | Fiji | NIH |